Thr80 of Sheep Podoplanin Is a Critical Epitope of the Antisheep Podoplanin Monoclonal Antibody: PMab-256
Copyright policy )Thr80 of Sheep Podoplanin Is a Critical Epitope of the Antisheep Podoplanin Monoclonal Antibody: PMab-256
An antisheep podoplanin (sPDPN) monoclonal antibody (mAb), PMab-256, has recently been established. PMab-256 shows positive immunostaining for lymphatic endothelial cells, lung type I alveolar cells, and kidney podocytes. PDPN possesses three platelet-aggregation-stimulating (PLAG) domains, PLAG1, PLAG2, and PLAG3, and a PLAG-like domain (PLD). The binding epitope of many anti-PDPN mAbs is located in PLAG domains or PLD. The purpose of this study is to determine the binding epitope of PMab-256. Analysis of sPDPN deletion mutants revealed that the N-terminus of the PMab-256 epitope exists between amino acids 75 and 80 of sPDPN. Furthermore, analysis of sPDPN point mutations demonstrated that the critical epitope includes Thr80 of sPDPN, indicating that the PMab-256 epitope is in the PLD of sPDPN.
- Tohoku University Japan
Membrane Glycoproteins, Sheep, Podocytes, Antibodies, Monoclonal, Endothelial Cells, CHO Cells, Epitopes, Cricetulus, Antibody Specificity, Cricetinae, Animals, Humans, Epitope Mapping
Membrane Glycoproteins, Sheep, Podocytes, Antibodies, Monoclonal, Endothelial Cells, CHO Cells, Epitopes, Cricetulus, Antibody Specificity, Cricetinae, Animals, Humans, Epitope Mapping
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