Monoclonal Antibody Against Membrane Protein of Transmissible Gastroenteritis Virus
Copyright policy )Monoclonal Antibody Against Membrane Protein of Transmissible Gastroenteritis Virus
Transmissible gastroenteritis virus (TGEV) is a porcine coronavirus that can cause piglet diarrhea with high mortality rates. TGEV membrane (M) protein not only plays a vital role in the process of virus assembly and budding, but also induces the production of interferon-α during infection. In this study, a monoclonal antibody (MAb) designated 7G7, against the TGEV M protein was generated by inoculating BALB/c mice with TGEV followed by hybridoma technique. Immunofluorescence assays indicated that MAb 7G7 was capable of detecting cell infection by TGEV. Virus-based ELISA demonstrated that MAb 7G7 can be used as a highly specific diagnostic reagent for TGEV.
- Jilin Agricultural University China (People's Republic of)
- Northeast Agricultural University China (People's Republic of)
Mice, Inbred BALB C, Hybridomas, Coronavirus M Proteins, Gastroenteritis, Transmissible, of Swine, Swine, Antibodies, Monoclonal, Enzyme-Linked Immunosorbent Assay, Viral Matrix Proteins, Mice, Antibody Specificity, Animals, Fluorescent Antibody Technique, Indirect, DNA Primers
Mice, Inbred BALB C, Hybridomas, Coronavirus M Proteins, Gastroenteritis, Transmissible, of Swine, Swine, Antibodies, Monoclonal, Enzyme-Linked Immunosorbent Assay, Viral Matrix Proteins, Mice, Antibody Specificity, Animals, Fluorescent Antibody Technique, Indirect, DNA Primers
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).2 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!