The fruit of Emblica officinalis has been used in the Ayurvedic system of medicine for the treatment of different ailments and is also an ingredient of various traditional medicinal herbal formulations in India and other countries. To investigate the protective effect of Emblica officinalis fruit extract (EFE) against alcohol-induced brain mitochondrial dysfunction, male Wistar rats were orally administered 20% alcohol (5 g/kg of body weight/day) and EFE (250 mg/kg of body weight/day) for 60 days. Alcohol-treated rats showed significantly lowered activities of mitochondrial antioxidant enzymes (superoxide dismutase and glutathione peroxidase) and reduced glutathione compared with those of experimental control rats. Furthermore, alcohol feeding lowered the activities of NADH dehydrogenase, succinate dehydrogenase (SDH), and cytochrome c oxidase and the content of cytochromes followed by increased levels of nitric oxide (NO), thiobarbituric acid-reactive substances, and protein carbonyls. No significant change was observed in membrane potential. Administration of EFE to alcohol-treated rats, lowered the levels of NO, protein carbonyls, and lipid peroxidation and elevated the activities of the antioxidant enzymes SDH, NADH dehydrogenase, and cytochrome c oxidase and the content of cytochromes. The active tannoid principles present in EFE with its antioxidant as well as NO scavenging properties might have contributed to the observed protection against alcohol-induced brain mitochondrial dysfunction.