Discrimination Between Protective and Enhancing HIV Antibodies
Copyright policy )Discrimination Between Protective and Enhancing HIV Antibodies
Most attempts to produce a vaccine against HIV-1 infection are utilizing envelope protein components. Hypothetically such vaccine candidates could stimulate production of antibodies that enhance HIV-1 infection via the macrophage route of entry and, consequently, cannot be detected in the conventional neutralization assay. To study this hypothesis we report an assay designed to evaluate the protective/enhancing activity of serum from seropositive immunized or infected individuals. Highly purified activated FcR-bearing monocytes-macrophages were infected with HIV-1 in the presence of the sera, then washed and cocultured with activated peripheral blood mononuclear cells (PBMC) from a normal donor. Productive viral infection, as evaluated by p24 antigen semiquantitative assay in the culture supernatants, allow evaluation of protective/enhancing activity of the sera. The data clearly show that protective rather than enhancing activity is present in the serum of env protein-immunized individuals.
- Institut Jean Godinot France
Acquired Immunodeficiency Syndrome, HIV Antigens, Macrophages, Viral Core Proteins, HIV Core Protein p24, Gene Products, gag, HIV Antibodies, Monocytes, Kinetics, Random Allocation, HIV Seropositivity, HIV-1, Leukocytes, Mononuclear, Humans, Cells, Cultured
Acquired Immunodeficiency Syndrome, HIV Antigens, Macrophages, Viral Core Proteins, HIV Core Protein p24, Gene Products, gag, HIV Antibodies, Monocytes, Kinetics, Random Allocation, HIV Seropositivity, HIV-1, Leukocytes, Mononuclear, Humans, Cells, Cultured
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