Abstract A method for the calibration of multidirectional spectroradiometers (MUDISs) capable of the simultaneous measurement of spectral radiance at different wavelengths is presented. The calibration of the spectroradiometer is challenging and crucial for high quality measurements of the angular dependence of the radiance. The calibration device consists of an integrating sphere (also known as Ulbrichtkugel), with a diameter of 100 cm, equipped with three 100 W lamps positioned in the lower hemisphere, with the input optics of the MUDIS directed towards the upper hemisphere. The MUDIS detects radiation from 113 different directions simultaneously in a wavelength range from 300 nm to 550 nm. Due to multiple reflections within the sphere, the radiance from the upper hemisphere is nearly homogeneous with deviations of less than 3% on average. Disregarding the 3% variability and assuming a homogeneous radiance inside the upper hemisphere of the integrating sphere, the spectral responsivities of all the MUDIS channels were determined based on the measured zenith radiance, which was detected by a pre-calibrated Network for the Detection of Atmospheric Composition Change reference spectroradiometer containing a scanning double monochromator with a unidirectional input optics. The input optics of the MUDIS contains thin fibers that should not be moved to avoid changes in the instrument’s responsivity. The proposed method is therefore suited to determine the absolute responsivity of the MUDIS for all directions.