Evidence of Porcine Endogenous Retroviruses in Porcine Factor VIII and Evaluation of Transmission to Recipients with Hemophilia
Copyright policy )Evidence of Porcine Endogenous Retroviruses in Porcine Factor VIII and Evaluation of Transmission to Recipients with Hemophilia
Since 1984, unheated porcine clotting factor VIII (Hyate:C) has been used to treat severe bleeding episodes in persons with hemophilia who have antibodies to human clotting factor. We document the presence of porcine endogenous retrovirus (PERV) in plasma samples of pigs and in clinical lots of Hyate:C. Both gag and pol PERV RNA sequences were detected by reverse-transcriptase (RT) polymerase chain reaction in 13 of 13 lots of Hyate:C tested. Among 10 of these lots, RT activity also was detected, which confirms the presence of retroviral particles. To assess the transmission of PERV to Hyate:C recipients, we tested serum specimens from 88 recipients of Hyate:C and 23 noninfused control subjects for anti-PERV antibodies by using a Western blot assay. None of the samples was positive. Our data document that PERV particles are a common contaminant of Hyate:C products and suggest that the risk of PERV transmission from these percutaneous exposures is very low.
- Centers for Disease Control and Prevention United States
- National Center for Emerging and Zoonotic Infectious Diseases United States
- Office of Infectious Diseases United States
Swine Diseases, Factor VIII, Reverse Transcriptase Polymerase Chain Reaction, Swine, Endogenous Retroviruses, Molecular Sequence Data, Antibodies, Viral, Hemophilia A, Plasma, Animals, Humans, RNA, Viral, Drug Contamination, Retroviridae Infections
Swine Diseases, Factor VIII, Reverse Transcriptase Polymerase Chain Reaction, Swine, Endogenous Retroviruses, Molecular Sequence Data, Antibodies, Viral, Hemophilia A, Plasma, Animals, Humans, RNA, Viral, Drug Contamination, Retroviridae Infections
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