In summary, (1) male sterility and vegetative retardation in emmer wheat with ovata cytoplasm is not changed in remote offspring; (2) pollen restoration by the nuclear genes of T. dicoccoides is not based on the alteration of the cytoplasmic factor itself; (3) vegetative retardation in ovata plants with durum cytoplasm is not changed in remote offspring; (4) durum cytoplasm is not affected by the ovata nucleus in respect to male-sterility; and (5) heterogenity in genome combination does not cause cytoplasmic alteration. From these results, it is concluded that cytoplasmic properties which are species specific are maintained unchanged through the crossing with different species.