An attempt is made to understand the physical and kinematic conditions in the M16 active star formation site, by means of mapping observations of neutral and ionized hydrogen and observations of (C-12)O from selected positions within the H II regions. The 21-cm maps suggest that at least two neutral clouds are present in the M16 complex, one of which is likely to be the remains of the cloud in which star formation initially occurred, and the other is probably the cold gas remaining from a disrupted cloud edge where the ionization front has crossed the cloud-intercloud boundary. Multislit echelle spectra of the H-alpha line are obtained to determine the physical conditions within the ionized gas, and H-alpha maps show that the ionized gas is systematically blueshifted in the east and splits into two streams in the southwest.