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The Journal of Cell Biology
Article . 1974 . Peer-reviewed
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AN ULTRASENSITIVE VIBRATING PROBE FOR MEASURING STEADY EXTRACELLULAR CURRENTS

Authors: L F, Jaffe; R, Nuccitelli;

AN ULTRASENSITIVE VIBRATING PROBE FOR MEASURING STEADY EXTRACELLULAR CURRENTS

Abstract

We describe a vibrating probe system for measuring relatively steady electrical current densities near individual living cells. It has a signal-to-noise ratio at least 100 times greater than previously available techniques. Thus it can be used to detect current densities as small as 10 nA/cm2 in serum when a 30-µm diameter probe is vibrated at 200 Hz between two points 30 µm apart, and the amplifier's time constant is set at 10 s. Moreover, it should be generally insensitive to interference by concentration gradients. It has been first used to reveal and study 100-s long current pulses which developing fucoid embryos drive through themselves.

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Keywords

Embryo, Nonmammalian, Heparin, Osmolar Concentration, Electric Conductivity, Dextrans, Sulfuric Acids, Embryo, Mammalian, Seaweed, Cell Physiological Phenomena, Electrophysiology, Evaluation Studies as Topic, Female, Protamines, Microelectrodes, Mathematics

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    selected citations
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    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    508
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Top 1%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Top 0.1%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Top 10%
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
508
Top 1%
Top 0.1%
Top 10%
bronze