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Inhibition of HCV Replication in HCV Replicon by shRNAs

descriptionPublicationkeyboard_double_arrow_right Article 01 Aug 2006 English Publisher:Informa UK LimitedJournal:Nucleosides, Nucleotides and Nucleic Acids, volume 25, pages 801-805 (issn: 1525-7770, eissn: 1532-2335,

Authors: Hiroshi Takaku; Naoko Miyano-Kurosaki; Hitoshi Takahashi; Hiroyuki Hamazaki; Kunitada Shimotohno;

doi: 10.1080/15257770600726091

pmid: 16898418

Inhibition of HCV Replication in HCV Replicon by shRNAs

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Abstract

We show that the vector-derived long dsRNA specifically inhibits the replication of HCV RNA in HCV replicon. We designed a long dsRNA targeted to the full-length HCV IRES/core elements (1-to 377-nt). Our results revealed that the replication of HCV RNA was reduced to near background levels in a sequence-specific manner by the long dsRNAs in the HCV replicon. We also designed four shRNAs against several regions (120- to 139-nt, 260- to 279-nt, 330- to 349-nt, and 340- to 359-nt) of the HCV IRES/Core elements. The two HCV IRES/core-specific shRNAs, 330- to 349-nt and 340- to 359-nt, containing the AUG initiation codon sequence showed stronger HCV inhibitory effects than the other two shRNAs, 120- to 139-nt and 260- to 279-nt.

Related Organizations

Kyoto University
Japan
Chiba Institute of Technology
Japan

Keywords

Replicon, Hepacivirus, Microbial Sensitivity Tests, Virus Replication, RNA, Double-Stranded

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