Background: Music therapy is a treatment which facilitates psychological and physiological relaxation. Psychological effects of music are reflected by physiological parameters.Objective: The aim of the study was to assess influence of a special composing relaxation piece of music on HRV and psychological area. Comparison of these areas may contribute to better selection of music for therapy.Methods: A total of 15 healthy women were tested by HRV (included VLF, LF, HF and LF/HF parameters) and by Natanson’s method, which tested four spheres of music perception: emotional, imaginative, psychomotorical, intellectual.Discussion: The study showed a tendency to a significant decrease of LF during and after listening to the music. HF, LF and LF/HF components reflected the nervous system during listening the music and correlated with psychological area. VLF component was associated with emotional sphere.Conclusions: The results showed that a piece of music was received as relaxation in the psychological and physi...