This work describes the chromatographic separation of trace amounts of Thorium (mg or less) from large quantities of Uranium (g or kg). The separation method employs extraction chromatographic materials containing tetra(n‐octyl)diglycolamide (TODGA) and quaternary amine extractants. Applications of this separation include isolation of 234Th from 238U and 229Th from 233U. 234Th could be a useful radiotracer, providing a continuous supply of radiothorium from natural or depleted uranium, while 229Th is the source material for 225Ac and 213Bi, radionuclides with promising applications in nuclear medicine.