Genomic sequencing.
Copyright policy )Genomic sequencing.
Unique DNA sequences can be determined directly from mouse genomic DNA. A denaturing gel separates by size mixtures of unlabeled DNA fragments from complete restriction and partial chemical cleavages of the entire genome. These lanes of DNA are transferred and UV-crosslinked to nylon membranes. Hybridization with a short 32P-labeled single-stranded probe produces the image of a DNA sequence "ladder" extending from the 3' or 5' end of one restriction site in the genome. Numerous different sequences can be obtained from a single membrane by reprobing. Each band in these sequences represents 3 fg of DNA complementary to the probe. Sequence data from mouse immunoglobulin heavy chain genes from several cell types are presented. The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
- Harvard University United States
570, Multidisciplinary, Base Sequence, 500, Nucleic Acid Hybridization, DNA, DNA Restriction Enzymes, DNA-Directed RNA Polymerases, Nucleic Acid Denaturation, Methylation, Mice, Genes, Animals, Immunoglobulin Heavy Chains
570, Multidisciplinary, Base Sequence, 500, Nucleic Acid Hybridization, DNA, DNA Restriction Enzymes, DNA-Directed RNA Polymerases, Nucleic Acid Denaturation, Methylation, Mice, Genes, Animals, Immunoglobulin Heavy Chains
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).9K popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 0.1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 0.01% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 0.01%
Citations provided by BIP!Popularity provided by BIP!