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Proceedings of the National Academy of Sciences
Article
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Proceedings of the National Academy of Sciences
Article . 1977 . Peer-reviewed
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Proceedings of the National Academy of Sciences
Article . 1977
Data sources: Europe PubMed Central
https://dx.doi.org/10.1073/pna...
Article
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A monomeric form of pyruvate kinase in human pyruvate kinase deficiency.

descriptionPublicationkeyboard_double_arrow_right Article 01 Feb 1977 English Publisher:Proceedings of the National Academy of SciencesJournal:Proceedings of the National Academy of Sciences, volume 74, pages 501-504 (issn: 0027-8424, eissn: 1091-6490, Copyright policy )
Authors: K, Adachi; P K, Ghory; T, Asakura; E, Schwartz;

doi: 10.1073/pnas.74.2.501

pmid: 15247

pmc: PMC392317

A monomeric form of pyruvate kinase in human pyruvate kinase deficiency.

Abstract

A mutant pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) from human erythrocytes which is easily separated into monomers and dimers by gel chromatography is described. Tht mutant enzyme shows almost the same pH optimum and thermostability as normal enzyme, but has a decreased stability on shaking with air, a decreased Km for phosphoenolpyruvate and a loss of allosteric properties. The apparent Km values for phosphoenolpyruvate of tetramers and monomers were the same. The tetrameric enzyme was slightly activated by fructose-1,6-diphosphate but the monomeric form was not. The tetrameric enzyme was found to dissociate spontaneously to dimeric and monomeric forms.

Keywords

Erythrocytes, Hot Temperature, Macromolecular Substances, Pyruvate Kinase, Genetic Variation, Hydrogen-Ion Concentration, Adenosine Diphosphate, Molecular Weight, Phosphoenolpyruvate, Kinetics, Drug Stability, Humans

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
8
Average
Average
Average
bronze