Plant diseases seriously damage crop production, and most plant diseases are caused by fungi. Fungal cell walls contain chitin, a highly conserved component that is widely recognised by plants as a PAMP (pathogen-associated molecular pattern) to induce defence responses. The molecular mechanisms that function downstream of chitin-triggered intracellular phosphorylation remain largely unknown. In this study, we performed quantitative phosphoproteomics analysis to study protein phosphorylation changes in the plasma membrane after chitin treatment in Arabidopsis thaliana L. seedlings. Proteins with altered phosphorylation status after chitin treatment participated in biological processes ranging from signalling, localisation, and transport, to biogenesis, processing, and metabolism, suggesting that PAMP signalling targets multiple processes to coordinate the immune response. These results provide important insights into the molecular mechanism of chitin-induced plant immunity.