Molecular evidence for the presence of huanglongbing in Pakistan

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2007 English Publisher:Springer Science and Business Media LLCJournal:Australasian Plant Disease Notes, volume 2, page 37 (issn: 1833-928X,

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Authors: Shahid Nadeem Chohan; Raheel Qamar; Irfan Sadiq; Maleeha Azam; Paul Holford; Andrew Beattie;

doi: 10.1071/dn07019

Molecular evidence for the presence of huanglongbing in Pakistan

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Abstract

The presence of Candidatus Liberibacter asiaticus in citrus orchards in the North-West Frontier Province of Pakistan has been confirmed. Samples of DNA extracted from leaves of putatively infected citrus plants and from the vector of the disease, Diaphorina citri, were subjected to analysis using PCR and produced DNA amplicons characteristic of this bacterium. To confirm the exact nature of the pathogen, amplicons were sequenced and the resulting data showed 100% identity with published sequences of the Ca. L. asiaticus rplKAJL-rpoBC operon.

Related Organizations

Western Sydney University
Australia
COMSATS University Islamabad
Pakistan

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