Previous reports have produced conflicting conclusions regarding the relationship among tooth agenesis, the congenital absence of teeth, and variations in craniofacial morphology. Traditional cephalometric assessment and computer-assisted morphometric analysis of jaw size were used to reexamine this relationship in a white orthodontic population. A positive diagnosis of tooth agenesis (including third molar determination) was made in 89 subjects of mixed age and sex who previously had orthodontic treatment but who were not determined to have a recognized syndrome or a craniofacial anomaly. Cephalograms for these subjects were compared with control data from subjects reported in Bolton Standards of Dentofacial Developmental Growth. Missing teeth and their locations were correlated with changes in selected linear and angular measurements of the Bolton cephalometric analysis. Area measurements of the jaw size of the subjects grouped by age were compared with Bolton cephalometric templates. Relatively little correlation was found between missing teeth and changes in cephalometric measurements. Where identified, a decreased maxillary jaw size and maxillary tooth agenesis were generally associated. Two-dimensional area measurements showed that most age groups had significant decreases in maxillary jaw size associated with tooth agenesis. Relatively few groups had significant changes in mandibular size associated with tooth agenesis.