Light Sheet Microscopy and Imaging introduces the fundamentals and important advancements at the frontiers of optical physics and applied sciences. It presents coherent, in-depth explanations of different variants of light sheet microscopes beginning from the basics of light. This emerging technology has an impact on a range of disciplines from nanophysics (nanolithography) to biophysics (imaging, cell biology, developmental biology, and biotechniques) and is destined to influence every field of science and engineering. This book provides an in-depth study of:Fundamentals of light sheet microscopy and important advancements in the technologyPrinciples of light sheet physics, optical imaging, computational techniques, and experimental design, with applications from applied physics to biological imagingInsights into a wide range of light sheet microscopy techniques, optical setups, and imaging science Light Sheet Microscopy and Imaging is an excellent resource for research scientists in optical microscopy and biomedical imaging, and for students studying imaging optics, biomedical engineering, light sheet microscopy, applied physics, and their applications.