
pmid: 12653942
AbstractA linkage map of the Ixodes scapularis genome was constructed, based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence‐Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 F1 intercross progeny from a single, field‐collected P1 female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of ≈ 109 bp, the relationship of physical to genetic distance was found to be ≈ 300 kb/cM in the I. scapularis genome.
Genetic Markers, Male, Genome, Ixodes, Chromosome Mapping, Genes, Insect, 630, Random Amplified Polymorphic DNA Technique, Animals, Female, Public Health, Crosses, Genetic, Polymorphism, Single-Stranded Conformational, Gene Library, Microsatellite Repeats, Sequence Tagged Sites
Genetic Markers, Male, Genome, Ixodes, Chromosome Mapping, Genes, Insect, 630, Random Amplified Polymorphic DNA Technique, Animals, Female, Public Health, Crosses, Genetic, Polymorphism, Single-Stranded Conformational, Gene Library, Microsatellite Repeats, Sequence Tagged Sites
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