descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 2000 English Publisher:WileyJournal:Insect Molecular Biology, volume 9, pages 263-268 (issn: 0962-1075, eissn: 1365-2583,

Authors: M. C. De Vicente; Rosa Gabarra; Nuria Agustí;

doi: 10.1046/j.1365-2583.2000.00185.x

pmid: 10886409

Developing SCAR markers to study predation on Trialeurodes vaporariorum

- Summary
- Subjects
- Related research
  (2)
- Metrics

Abstract

AbstractDNA markers of Trialeurodes vaporariorum were developed to detect remains of these whitefly in the gut of the predator Dicyphus tamaninii. A 2400‐bp DNA fragment of T. vaporariorum, absent in other closely related prey species and in the predator banding pattern, was identified by random amplified polymorphic DNA (RAPD) analysis. After cloning and sequencing this fragment, two pairs of sequence‐characterized amplified region (SCAR) primers were developed, amplifying single bands of 2100 bp and 310 bp, respectively. Detection of T. vaporariorum DNA in the predator gut was only possible using the primers that amplified the shortest fragment. Specificity tests performed with this pair of primers showed the presence of the 310‐bp band for T. vaporariorum in all stages.

Related Organizations

Institute for Research and Technology in Food and Agriculture
Spain

Keywords

Genetic Markers, DNA, Complementary, Polymorphism, Genetic, Base Sequence, Molecular Sequence Data, Genes, Insect, Sensitivity and Specificity, Random Amplified Polymorphic DNA Technique, Hemiptera, Heteroptera, Predatory Behavior, Animals, Digestive System

2 Research products, page 1 of 1

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	53
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%