Construction of a [NiFe]-hydrogenase deletion mutant of Desulfovibrio vulgaris Hildenborough

descriptionPublicationkeyboard_double_arrow_right Article 01 Feb 2005 English Publisher:Portland Press Ltd.Journal:Biochemical Society Transactions, volume 33, pages 59-60 (issn: 0300-5127, eissn: 1470-8752,

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Authors: A, Goenka; J K, Voordouw; W, Lubitz; W, Gärtner; G, Voordouw;

doi: 10.1042/bst0330059

pmid: 15667264

Construction of a [NiFe]-hydrogenase deletion mutant of Desulfovibrio vulgaris Hildenborough

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Abstract

A mutant of Desulfovibrio vulgaris Hildenborough lacking a gene for [NiFe] hydrogenase was generated. Growth studies, performed for the mutant in comparison with the wild-type, showed no strong differences during the exponential growth phase. However, the mutant cells died more rapidly in the stationary growth phase.

Related Organizations

Max Planck Society
Germany
University of Calgary
Canada

Keywords

Blotting, Southern, Base Sequence, Hydrogenase, Blotting, Western, Desulfovibrio vulgaris, DNA Primers

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