Incorporation of oxygen-18 into the 25-position of cholecalciferol by hepatic cholecalciferol 25-hydroxylase
Copyright policy )Incorporation of oxygen-18 into the 25-position of cholecalciferol by hepatic cholecalciferol 25-hydroxylase
The oxygen enzymically inserted as a hydroxy function by rat liver post-mitochondrial fraction into the 25-position of cholecalciferol to giver 25-hydroxycholecaliferol is derived exclusively from molecular O2. Therefore like the other two cholecalciferol hydroxylases, i.e. 25-hydroxycholecalciferol 1alpha-hydroxylase and 25-hydroxycholecalciferol 24-hydroxylase, the cholecalciferol 25-hydroxylase is also a mono-oxygenase (‘mixed-function oxidase’).
- University of Wisconsin System United States
- University of Wisconsin–Oshkosh United States
Male, Hydroxycholecalciferols, Hydroxylation, Mass Spectrometry, Rats, Liver, Oxygen Radioisotopes, Steroid Hydroxylases, Animals, Female, Chromatography, High Pressure Liquid, Cholecalciferol
Male, Hydroxycholecalciferols, Hydroxylation, Mass Spectrometry, Rats, Liver, Oxygen Radioisotopes, Steroid Hydroxylases, Animals, Female, Chromatography, High Pressure Liquid, Cholecalciferol
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