The choline acetyltransferase of human placenta
Copyright policy )The choline acetyltransferase of human placenta
1. Various methods for the extraction of choline acetyltransferase (acetyl-CoA-choline O-acetyltransferase, EC 2.3.1.6) from immature human placenta (18-28 weeks of gestation) are described. 2. The crude enzyme was found to be stable at -18 degrees and +4 degrees under a variety of conditions. 3. Purification methods, including ammonium sulphate fractionation, gel filtration on various grades of Sephadex and DEAE-Sephadex fractionation, have yielded a preparation of high specific activity.
- Institute of Animal Physiology Slovakia
- Slovak Academy of Sciences Slovakia
Placenta, Chromatography, Gel, Humans, Female, In Vitro Techniques, Acetylcholine, Acyltransferases, Chemistry Techniques, Analytical
Placenta, Chromatography, Gel, Humans, Female, In Vitro Techniques, Acetylcholine, Acyltransferases, Chemistry Techniques, Analytical
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