The application of green‐synthesis principles is one of the most impressive research fields for the production of nanoparticles. Different kinds of biological systems have been used for this purpose. In the present study, AuNPs (gold nanoparticles) were prepared within a short time period using a fresh cell extract of the marine microalga Tetraselmis suecica as a reducing agent of HAuCl4 (chloroauric acid) solution. The UV–visible spectrum of the aqueous medium containing AuNPs indicated a peak at 530 nm, corresponding to the surface plasmon absorbance of AuNPs. The X‐ray diffraction pattern also showed a Bragg reflection related to AuNPs. Fourier‐transform infrared spectroscopy was performed for analysis of surface functional groups of AuNPs. Transmission electron microscopy and particle‐size‐distribution patterns determined by the laser‐light‐scattering method confirmed the formation of well‐dispersed AuNPs. The most frequent size of particles was 79 nm.