Profiling of RNA modifications by multiplexed stable isotope labelling

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2014 English Publisher:Royal Society of Chemistry (RSC)Journal:Chemical Communications, volume 50, page 3,516 (issn: 1359-7345, eissn: 1364-548X,

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Authors: Stefanie Kellner; Jennifer Neumann; David Rosenkranz; Svetlana Lebedeva; René F. Ketting; Hans Zischler; Dirk Schneider; +1 Authors

doi: 10.1039/c3cc49114e

pmid: 24567952

Profiling of RNA modifications by multiplexed stable isotope labelling

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Abstract

The combination of (15)N/(13)C stable isotope labelling (SIL) and LC-MS/MS revealed a total of 52 modifications in RNA from E. coli and yeast, including 10 previously undescribed modifications. Two modifications, N-ribosylnicotinamide and 2-methylthioadenosine, were newly detected in species hitherto thought not to contain these modifications.

Related Organizations

Johannes Gutenberg University of Mainz
Germany
Institute of Molecular Biology
Germany

Keywords

Tandem Mass Spectrometry, Isotope Labeling, Escherichia coli, RNA, Saccharomyces cerevisiae, Chromatography, Liquid

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