Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides
Copyright policy )Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides
Enzymatic recognition of unlocked nucleic acid (UNA) nucleotides was successfully accomplished. Therminator DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of a 81 mer UNA-modified DNA library was efficiently achieved by KOD DNA polymerase.
- University of Queensland Australia
- University of Queensland Australia
- University of Southern Denmark Denmark
- University of Queensland Australia
- Murdoch University Australia
Multidisciplinary, Base Sequence, 1503 Catalysis, 2504 Electronic, Molecular Sequence Data, 2506 Metals and Alloys, 2503 Ceramics and Composites, DNA-Directed DNA Polymerase, Aptamers, Nucleotide, 1600 Chemistry, Polymerase Chain Reaction, Substrate Specificity, Coatings and Films, Thermococcus, Chemistry, Nucleic Acid Conformation, 2508 Surfaces, Optical and Magnetic Materials, 2505 Materials Chemistry, DNA Primers, Gene Library
Multidisciplinary, Base Sequence, 1503 Catalysis, 2504 Electronic, Molecular Sequence Data, 2506 Metals and Alloys, 2503 Ceramics and Composites, DNA-Directed DNA Polymerase, Aptamers, Nucleotide, 1600 Chemistry, Polymerase Chain Reaction, Substrate Specificity, Coatings and Films, Thermococcus, Chemistry, Nucleic Acid Conformation, 2508 Surfaces, Optical and Magnetic Materials, 2505 Materials Chemistry, DNA Primers, Gene Library
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