Tailoring silver nanodots for intracellular staining
Copyright policy )Tailoring silver nanodots for intracellular staining
Through tailored oligonucleotide scaffolds, Ag nanocluster syntheses have yielded thermally and cell-culture medium stable silver cluster-based emitters. Optimizing ssDNA stability has enabled creation of highly concentrated and spectrally pure nanocluster emitters with strong intracellular emission. Both fixed and live-cell staining become possible, and intracellular delivery is demonstrated both through conjugation to cell-penetrating peptides and via microinjection.
- Emory University United States
- Parker H. Petit Institute for Bioengineering & Bioscience United States
- Georgia Institute of Technology United States
Mice, Microscopy, Confocal, Silver, Staining and Labeling, NIH 3T3 Cells, Animals, DNA, Single-Stranded, Metal Nanoparticles, Spectrophotometry, Ultraviolet, Cell-Penetrating Peptides
Mice, Microscopy, Confocal, Silver, Staining and Labeling, NIH 3T3 Cells, Animals, DNA, Single-Stranded, Metal Nanoparticles, Spectrophotometry, Ultraviolet, Cell-Penetrating Peptides
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