
MUC1 glycopeptide was efficiently coupled to glycosylphosphatidylinositol (GPI) derivatives by sortase A (SrtA), verifying that SrtA can accept sterically hindered glycopeptide as substrate for ligation with GPIs. This work has established a practical method for the chemoenzymatic synthesis of GPI-linked glycopeptides.
Cysteine Endopeptidases, Bacterial Proteins, Carbohydrate Sequence, Glycosylphosphatidylinositols, Molecular Sequence Data, Mucin-1, Biocatalysis, Glycopeptides, Amino Acid Sequence, Aminoacyltransferases
Cysteine Endopeptidases, Bacterial Proteins, Carbohydrate Sequence, Glycosylphosphatidylinositols, Molecular Sequence Data, Mucin-1, Biocatalysis, Glycopeptides, Amino Acid Sequence, Aminoacyltransferases
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