Cholesterol immobilization via ether-linked Sepharose gels

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1992 English Publisher:Royal Society of Chemistry (RSC)Journal:The Analyst, volume 117, page 953 (issn: 0003-2654, eissn: 1364-5528,

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Authors: R K, Satsangi; G E, Mott;

doi: 10.1039/an9921700953

pmid: 1503232

Cholesterol immobilization via ether-linked Sepharose gels

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Abstract

Cholesterol has been immobilized on Sepharose-6B via oxyether linkages to the 3- or 25-position. The 3- or 25-hydroxysterol methanesulfonates were coupled with epoxy-Sepharose-6B at 80 degrees C for 24 h. Approximately 2% of the ligand was incorporated into the gel. These types of affinity columns may be useful in purifying proteins that specifically bind or metabolize cholesterol.

Related Organizations

The University of Texas Health Science Center at Houston
United States
The University of Texas Health Science Center at San Antonio
United States

Keywords

Cholesterol, Sepharose, Gels, Ethers

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