There has been significant growth in the field of nanofluidics, where nanoscale analytical instruments employ micromachined features and are able to manipulate fluid samples with high precision and efficiency and have many advantages over their conventional (larger) analogues. The new edition of Nanofluidics has been fully revised and updated with the latest advancements and applications. With a focus on bioanalysis, specific applications are given with case studies. The end of each chapter now also features a methodology section to explain experimental protocols and “tips and tricks”. The editors draw on an international authorship and provide a handbook for the community. Written at an accessible level the book is suitable for both experts and non-experts alike.