Superficial vimentin mediates DENV-2 infection of vascular endothelial cells
Copyright policy )Superficial vimentin mediates DENV-2 infection of vascular endothelial cells
AbstractDamage to vascular endothelial cells (VECs) is a critical hallmark of hemorrhagic diseases caused by dengue virus (DENV). However, the precise molecular event involved in DENV binding and infection of VECs has yet to be clarified. In this study, vimentin (55 kDa) was identified to be involved in DENV-2 adsorption into VECs. This protein is located on the surface of VECs and interacts with DENV-2 envelope protein domain III (EDIII). The expression level of the superficial vimentin on VECs was not affected by viral infection or siRNA interference, indicating that the protein exists in a particular mode. Furthermore, the rod domain of the vimentin protein mainly functions in DENV-2 adsorption into VECs. Molecular docking results predicted several residues in vimentin rod and DENV EDIII; these residues may be responsible for cell–virus interactions. We propose that the superficial vimentin could be a novel molecule involved in DENV binding and infection of VECs. DENV EDIII directly interacts with the rod domain of vimentin on the VEC surface and thus mediates the infection.
- Third Military Medical University China (People's Republic of)
Protein Conformation, alpha-Helical, Binding Sites, Genetic Vectors, Endothelial Cells, Gene Expression, Dengue Virus, Article, Recombinant Proteins, Cell Line, Molecular Docking Simulation, Aedes, Chlorocebus aethiops, Escherichia coli, Animals, Humans, Receptors, Virus, Protein Conformation, beta-Strand, Protein Interaction Domains and Motifs, Cloning, Molecular, Vero Cells, Protein Binding
Protein Conformation, alpha-Helical, Binding Sites, Genetic Vectors, Endothelial Cells, Gene Expression, Dengue Virus, Article, Recombinant Proteins, Cell Line, Molecular Docking Simulation, Aedes, Chlorocebus aethiops, Escherichia coli, Animals, Humans, Receptors, Virus, Protein Conformation, beta-Strand, Protein Interaction Domains and Motifs, Cloning, Molecular, Vero Cells, Protein Binding
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).59 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!