Hexokinase I N-terminal based peptide prevents the VDAC1-SOD1 G93A interaction and re-establishes ALS cell viability
Copyright policy )Hexokinase I N-terminal based peptide prevents the VDAC1-SOD1 G93A interaction and re-establishes ALS cell viability
AbstractSuperoxide Dismutase 1 mutants associate with 20–25% of familial Amyotrophic Lateral Sclerosis (ALS) cases, producing toxic aggregates on mitochondria, notably in spinal cord. The Voltage Dependent Anion Channel isoform 1 (VDAC1) in the outer mitochondrial membrane is a docking site for SOD1 G93A mutant in ALS mice and the physiological receptor of Hexokinase I (HK1), which is poorly expressed in mouse spinal cord. Our results demonstrate that HK1 competes with SOD1 G93A for binding VDAC1, suggesting that in ALS spinal cord the available HK1-binding sites could be used by SOD1 mutants for docking mitochondria, producing thus organelle dysfunction. We tested this model by studying the action of a HK1-N-terminal based peptide (NHK1). This NHK1 peptide specifically interacts with VDAC1, inhibits the SOD1 G93A binding to mitochondria and restores the viability of ALS model NSC34 cells. Altogether, our results suggest that NHK1 peptide could be developed as a therapeutic tool in ALS, predicting an effective role also in other proteinopathies.
- University of Catania Italy
- National Research Council Italy
- Institute of Crystallography Italy
flow citometry, VDAC1-SOD1 G93A interaction; ALS cell viability; Hexokinase 1 N-terminal, Cell Survival, Binding, Competitive, Article, Cell Line, copper zinc superoxide dismutase, Mice, Superoxide Dismutase-1, Hexokinase, molecular biology, Animals, Humans, Molecular Targeted Therapy, Membrane Potential, Mitochondrial, Motor Neurons, Binding Sites, VDAC, Voltage-Dependent Anion Channel 1, Amyotrophic Lateral Sclerosis, Peptide Fragments, Mitochondria, mitochondria, Mutation, ALS
flow citometry, VDAC1-SOD1 G93A interaction; ALS cell viability; Hexokinase 1 N-terminal, Cell Survival, Binding, Competitive, Article, Cell Line, copper zinc superoxide dismutase, Mice, Superoxide Dismutase-1, Hexokinase, molecular biology, Animals, Humans, Molecular Targeted Therapy, Membrane Potential, Mitochondrial, Motor Neurons, Binding Sites, VDAC, Voltage-Dependent Anion Channel 1, Amyotrophic Lateral Sclerosis, Peptide Fragments, Mitochondria, mitochondria, Mutation, ALS
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