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Oil-free hyaluronic acid matrix for serial femtosecond crystallography

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 18 Apr 2016 Japan, Korea (Republic of) English Publisher:Springer Science and Business Media LLCJournal:Scientific Reports, volume 6 (eissn: 2045-2322, Copyright policy )
Authors: Sugahara, M; Song, C; Suzuki, M; Masuda, T; Inoue, S; Nakane, T; Yumoto, F; +10 Authors

doi: 10.1038/srep24484

pmid: 27087008

pmc: PMC4834484

handle: 2433/210352

Oil-free hyaluronic acid matrix for serial femtosecond crystallography

Abstract

AbstractThe grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-Å resolution.

Countries
Japan, Korea (Republic of)
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Keywords

PROTEIN, Crystallography, X-Ray, Article, FREE-ELECTRON LASER, X-RAY-DIFFRACTION, Muramidase, Endopeptidase K, Hyaluronic Acid, Oils, SYSTEM, SACLA

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    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
48
Top 10%
Top 10%
Top 10%
Green
gold