TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton
Copyright policy )TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton
AbstractCurrent methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton, concurrently with host gene expression, using TRIzol-based DNA/RNA extraction and Alu sequence qPCR amplification. This approach enables precise quantification of tumor cells and corresponding host gene expression during metastatic colonization in xenograft models.
- Vanderbilt University United States
- United States Department of Veterans Affairs United States
Genetic Markers, Gene Expression Profiling, Mice, Nude, Reproducibility of Results, Bone Neoplasms, DNA, Neoplasm, Real-Time Polymerase Chain Reaction, Guanidines, Sensitivity and Specificity, Article, Mice, Phenols, Alu Elements, Cell Line, Tumor, Biomarkers, Tumor, Animals, RNA, Neoplasm
Genetic Markers, Gene Expression Profiling, Mice, Nude, Reproducibility of Results, Bone Neoplasms, DNA, Neoplasm, Real-Time Polymerase Chain Reaction, Guanidines, Sensitivity and Specificity, Article, Mice, Phenols, Alu Elements, Cell Line, Tumor, Biomarkers, Tumor, Animals, RNA, Neoplasm
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