Pathogenic diversity amongst serotype C VGIII and VGIV Cryptococcus gattii isolates
Copyright policy )Pathogenic diversity amongst serotype C VGIII and VGIV Cryptococcus gattii isolates
AbstractCryptococcus gattii is one of the causative agents of human cryptococcosis. Highly virulent strains of serotype B C. gattii have been studied in detail, but little information is available on the pathogenic properties of serotype C isolates. In this study, we analyzed pathogenic determinants in three serotype C C. gattii isolates (106.97, ATCC 24066 and WM 779). Isolate ATCC 24066 (molecular type VGIII) differed from isolates WM 779 and 106.97 (both VGIV) in capsule dimensions, expression of CAP genes, chitooligomer distribution and induction of host chitinase activity. Isolate WM 779 was more efficient than the others in producing pigments and all three isolates had distinct patterns of reactivity with antibodies to glucuronoxylomannan. This great phenotypic diversity reflected in differential pathogenicity. VGIV isolates WM 779 and 106.97 were similar in their ability to cause lethality and produced higher pulmonary fungal burden in a murine model of cryptococcosis, while isolate ATCC 24066 (VGIII) was unable to reach the brain and caused reduced lethality in intranasally infected mice. These results demonstrate a high diversity in the pathogenic potential of isolates of C. gattii belonging to the molecular types VGIII and VGIV.
- University of Sydney Australia
- Federal University of Rio de Janeiro Brazil
- Oswaldo Cruz Foundation Brazil
- Federal University of Rio Grande do Sul Brazil
- Universidade Federal do Rio Grande do Sul Brazil
Mice, Inbred BALB C, Chitinases, Brain, Cryptococcus gattii, Cryptococcosis, Serogroup, Article, Survival Rate, Disease Models, Animal, Mice, Phenotype, Polysaccharides, Animals, Humans, Female, Lung, Bacterial Capsules
Mice, Inbred BALB C, Chitinases, Brain, Cryptococcus gattii, Cryptococcosis, Serogroup, Article, Survival Rate, Disease Models, Animal, Mice, Phenotype, Polysaccharides, Animals, Humans, Female, Lung, Bacterial Capsules
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