An activity-dependent switch to cap-independent translation triggered by eIF4E dephosphorylation

descriptionPublicationkeyboard_double_arrow_right Article 18 Feb 2003 English Publisher:Springer Science and Business Media LLCJournal:Nature Neuroscience, volume 6, pages 219-220 (issn: 1097-6256, eissn: 1546-1726,

Copyright policy )Funded by:NIH | CELLULAR MECHANISMS OF NE..., CIHR | unidentified

Authors: John R, Dyer; Stephan, Michel; Wenjau, Lee; Vincent F, Castellucci; Nancy L, Wayne; Wayne S, Sossin;

doi: 10.1038/nn1018

pmid: 12592407

An activity-dependent switch to cap-independent translation triggered by eIF4E dephosphorylation

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Abstract

The rate of translation of egg-laying hormone (ELH) in Aplysia californica bag cell neurons rises after an afterdischarge (AD), the physiological trigger for egg-laying1. We found that the 5′ untranslated region (5′ UTR) of ELH possessed an internal ribosomal entry site (IRES), and that an AD was accompanied by a switch to cap-independent, IRES-mediated translation, indicating that the use of a cellular IRES can be regulated by physiological activity. Furthermore, the AD caused dephosphorylation of the initiation factor eIF4E, which was sufficient to increase use of the ELH IRES, showing that dephosphorylation of eIF4E can trigger a switch to IRES-mediated translation.

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Keywords

Neurons, Binding Sites, Invertebrate Hormones, Ganglia, Invertebrate, Luminescent Proteins, Eukaryotic Initiation Factor-4E, Protein Biosynthesis, Aplysia, Animals, RNA, Messenger, Phosphorylation, 5' Untranslated Regions, Ribosomes

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