
doi: 10.1038/nmeth1108
pmid: 17952088
We report stimulated emission depletion (STED) fluorescence microscopy with continuous wave (CW) laser beams. Lateral fluorescence confinement from the scanning focal spot delivered a resolution of 29-60 nm in the focal plane, corresponding to a 5-8-fold improvement over the diffraction barrier. Axial spot confinement increased the axial resolution by 3.5-fold. We observed three-dimensional (3D) subdiffraction resolution in 3D image stacks. Viable for fluorophores with low triplet yield, the use of CW light sources greatly simplifies the implementation of this concept of far-field fluorescence nanoscopy.
ddc:610, Optics and Photonics, Microscopy, Confocal, Nuclear Lamina, Qa-SNARE Proteins, Lasers, Immunohistochemistry, PC12 Cells, Lamins, Microspheres, Rats, Imaging, Three-Dimensional, Spectrometry, Fluorescence, Microscopy, Fluorescence, Neurofilament Proteins, Cell Line, Tumor, Animals, Humans, Fluorescent Dyes
ddc:610, Optics and Photonics, Microscopy, Confocal, Nuclear Lamina, Qa-SNARE Proteins, Lasers, Immunohistochemistry, PC12 Cells, Lamins, Microspheres, Rats, Imaging, Three-Dimensional, Spectrometry, Fluorescence, Microscopy, Fluorescence, Neurofilament Proteins, Cell Line, Tumor, Animals, Humans, Fluorescent Dyes
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