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Nature Methods
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Nature Methods
Article . 2015 . Peer-reviewed
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https://dx.doi.org/10.1038/nme...
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DNA library construction using Gibson Assembly®

descriptionPublicationkeyboard_double_arrow_right Article 29 Oct 2015 English Publisher:Springer Science and Business Media LLCJournal:Nature Methods, volume 12, pages i-ii (issn: 1548-7091, eissn: 1548-7105, Copyright policy )
Authors: Steven Thomas; Nathaniel D Maynard; John Gill;

doi: 10.1038/nmeth.f.384

DNA library construction using Gibson Assembly®

Abstract

Since its introduction 6 years ago, the Gibson Assembly method has been widely adopted as a preferred cloning method. Here we describe an application of Gibson Assembly beyond routine cloning: assembly in DNA library construction. Advantages of using Gibson Assembly, specifically the SGI-DNA Gibson Assembly HiFi 1 Step kit, in library construction include speed, efficiency, scarless assembly with vector and versatility.

  • BIP!
    Impact byBIP!
    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 38
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Average
Powered by OpenAIRE graph
Found an issue? Give us feedback
selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
38
Top 10%
Top 10%
Average
bronze