High-throughput, image-based screening of pooled genetic-variant libraries
Copyright policy )High-throughput, image-based screening of pooled genetic-variant libraries
We report a high-throughput screening method that allows diverse genotypes and corresponding phenotypes to be imaged in individual cells. We achieve genotyping by introducing barcoded genetic variants into cells as pooled libraries and reading the barcodes out using massively multiplexed fluorescence in situ hybridization. To demonstrate the power of image-based pooled screening, we identified brighter and more photostable variants of the fluorescent protein YFAST among 60,000 variants.
- Harvard University United States
- Howard Hughes Medical Institute United States
570, High-Throughput Nucleotide Sequencing, Reproducibility of Results, Article, 576, Mutation, Escherichia coli, DNA Barcoding, Taxonomic, In Situ Hybridization, Fluorescence, Gene Library
570, High-Throughput Nucleotide Sequencing, Reproducibility of Results, Article, 576, Mutation, Escherichia coli, DNA Barcoding, Taxonomic, In Situ Hybridization, Fluorescence, Gene Library
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).67 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!