Plasmid-based one-pot saturation mutagenesis
Copyright policy )Plasmid-based one-pot saturation mutagenesis
Deep mutational scanning is a foundational tool for addressing the functional consequences of large numbers of mutants, but a more efficient and accessible method for construction of user-defined mutagenesis libraries is needed. Here we present nicking mutagenesis, a robust, single-day, one-pot saturation mutagenesis method performed on routinely prepped plasmid dsDNA. The method can be used to produce comprehensive or single- or multi-site saturation mutagenesis libraries.
- Michigan State University United States
- Northwestern University United States
DNA, DNA Restriction Enzymes, Sequence Analysis, DNA, beta-Lactamases, Amidohydrolases, Genes, Bacterial, Mutation, Pseudomonas aeruginosa, Escherichia coli, Mutagenesis, Site-Directed, DNA Breaks, Single-Stranded, Gene Library, Plasmids
DNA, DNA Restriction Enzymes, Sequence Analysis, DNA, beta-Lactamases, Amidohydrolases, Genes, Bacterial, Mutation, Pseudomonas aeruginosa, Escherichia coli, Mutagenesis, Site-Directed, DNA Breaks, Single-Stranded, Gene Library, Plasmids
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).153 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!