irCLIP platform for efficient characterization of protein–RNA interactions
Copyright policy )irCLIP platform for efficient characterization of protein–RNA interactions
The complexity of transcriptome-wide protein-RNA interaction networks is incompletely understood. While emerging studies are greatly expanding the known universe of RNA-binding proteins, methods for the discovery and characterization of protein-RNA interactions remain resource intensive and technically challenging. Here we introduce a UV-C crosslinking and immunoprecipitation platform, irCLIP, which provides an ultraefficient, fast, and nonisotopic method for the detection of protein-RNA interactions using far less material than standard protocols.
- Stanford University United States
- Veterans Health Administration United States
Binding Sites, DNA, Complementary, Ultraviolet Rays, High-Throughput Nucleotide Sequencing, RNA-Binding Proteins, Photochemical Processes, Sensitivity and Specificity, Cross-Linking Reagents, Humans, Immunoprecipitation, Transcriptome, HeLa Cells
Binding Sites, DNA, Complementary, Ultraviolet Rays, High-Throughput Nucleotide Sequencing, RNA-Binding Proteins, Photochemical Processes, Sensitivity and Specificity, Cross-Linking Reagents, Humans, Immunoprecipitation, Transcriptome, HeLa Cells
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