Cas9 gRNA engineering for genome editing, activation and repression
Copyright policy )Cas9 gRNA engineering for genome editing, activation and repression
We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.
- Wyss Institute for Biologically Inspired Engineering United States
- Massachusetts Institute of Technology United States
- Broad Institute United States
- BBN Technologies United States
- Harvard University United States
580, 570, Binding Sites, Genome, Transcription, Genetic, CRISPR-Associated Proteins, Genetic Vectors, RNA, Guide, CRISPR-Cas Systems, Flow Cytometry, HEK293 Cells, Microscopy, Fluorescence, Genes, Reporter, Mutagenesis, Mutation, Humans, Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Genetics, RNA Editing, CRISPR-Cas Systems, Genetic Engineering, Gene Deletion, Fluorescent Dyes
580, 570, Binding Sites, Genome, Transcription, Genetic, CRISPR-Associated Proteins, Genetic Vectors, RNA, Guide, CRISPR-Cas Systems, Flow Cytometry, HEK293 Cells, Microscopy, Fluorescence, Genes, Reporter, Mutagenesis, Mutation, Humans, Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Genetics, RNA Editing, CRISPR-Cas Systems, Genetic Engineering, Gene Deletion, Fluorescent Dyes
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