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Nature Methods
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Nature Methods
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Nature Methods
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https://dx.doi.org/10.1038/nme...
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Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy

descriptionPublicationkeyboard_double_arrow_right Article 29 May 2008 English Publisher:Springer Science and Business Media LLCJournal:Nature Methods, volume 5, pages 491-505 (issn: 1548-7091, eissn: 1548-7105, Copyright policy )
Authors: Keir C, Neuman; Attila, Nagy;

doi: 10.1038/nmeth.1218

pmid: 18511917

pmc: PMC3397402

Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy

Abstract

Single-molecule force spectroscopy has emerged as a powerful tool to investigate the forces and motions associated with biological molecules and enzymatic activity. The most common force spectroscopy techniques are optical tweezers, magnetic tweezers and atomic force microscopy. Here we describe these techniques and illustrate them with examples highlighting current capabilities and limitations.

Related Organizations
Keywords

Neodymium, Radiation, Optical Tweezers, Biophysics, Microscopy, Atomic Force, Kinetics, Magnetics, Spectrophotometry, Calibration, Escherichia coli, Biotechnology

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    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Top 0.1%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
2K
Top 0.1%
Top 0.1%
Top 0.1%
bronze