Photo-lysine captures proteins that bind lysine post-translational modifications
Copyright policy )Photo-lysine captures proteins that bind lysine post-translational modifications
Post-translational modifications (PTMs) have key roles in regulating protein-protein interactions in living cells. However, it remains a challenge to identify these PTM-mediated interactions. Here we develop a new lysine-based photo-reactive amino acid, termed photo-lysine. We demonstrate that photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine PTMs, including 'readers' and 'erasers' of histone modifications.
- University of Hong Kong China (People's Republic of)
- University of Hong Kong (香港大學) China (People's Republic of)
Models, Molecular, Light, Molecular Structure, Lysine, Histones, Diazomethane, Animals, Humans, Click Chemistry, Protein Processing, Post-Translational, HeLa Cells, Protein Binding
Models, Molecular, Light, Molecular Structure, Lysine, Histones, Diazomethane, Animals, Humans, Click Chemistry, Protein Processing, Post-Translational, HeLa Cells, Protein Binding
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