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An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 21 Apr 2010 English Publisher:Springer Science and Business Media LLCJournal:Nature, volume 465, pages 106-109 (issn: 0028-0836, eissn: 1476-4687, Copyright policy )Funded by:UKRI | SCORE: Supply Chain Optim...
Authors: Gao, Zhihuan; Liu, Hai-Liang; Daxinger, Lucia; Pontes, Olga; He, Xinjian; Qian, Weiqiang; Lin, Huixin; +12 Authors

doi: 10.1038/nature09025

pmid: 20410883

pmc: PMC2865564

handle: 10754/325291

An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation

Abstract

DNA methylation is an important epigenetic mark in many eukaryotes. In plants, 24-nucleotide small interfering RNAs (siRNAs) bound to the effector protein, Argonaute 4 (AGO4), can direct de novo DNA methylation by the methyltransferase DRM2 (refs 2, 4-6). Here we report a new regulator of RNA-directed DNA methylation (RdDM) in Arabidopsis: RDM1. Loss-of-function mutations in the RDM1 gene impair the accumulation of 24-nucleotide siRNAs, reduce DNA methylation, and release transcriptional gene silencing at RdDM target loci. RDM1 encodes a small protein that seems to bind single-stranded methyl DNA, and associates and co-localizes with RNA polymerase II (Pol II, also known as NRPB), AGO4 and DRM2 in the nucleus. Our results indicate that RDM1 is a component of the RdDM effector complex and may have a role in linking siRNA production with pre-existing or de novo cytosine methylation. Our results also indicate that, although RDM1 and Pol V (also known as NRPE) may function together at some RdDM target sites in the peri-nucleolar siRNA processing centre, Pol II rather than Pol V is associated with the RdDM effector complex at target sites in the nucleoplasm.

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Keywords

argonaute protein, polymerase chain reaction, Arabidopsis, [SDV.GEN] Life Sciences [q-bio]/Genetics, [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, gene silencing, Gene Expression Regulation, Plant, [SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics, eukaryote, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, cytosine, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [INFO.INFO-BI] Computer Science [cs]/Bioinformatics [q-bio.QM], DNA methylation, [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], Eukaryota, nucleotide, protein function, DNA-Binding Proteins, RNA, Plant, Argonaute Proteins, DNA RNA hybridization, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], RNA polymerase II, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, genetic marker, RNA Polymerase II, gene locus, [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], protein DNA binding, Article, loss of function mutation, [SDV.BID.SPT] Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BID.EVO] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], controlled study, Gene Silencing, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Cell Nucleus, single stranded DNA, Arabidopsis Proteins, Plant, Methyltransferases, DNA Methylation, small interfering RNA, nucleic acid, enzyme, RNA processing, Gene Expression Regulation, [SDV.BBM.MN] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], herb, Mutation, RNA, methyltransferase, methylation, mutation, protein

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
233
Top 1%
Top 1%
Top 1%
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