Investigations utilizing the isolated perfused tubule technique have greatly enhanced our understanding of normal renal physiology. Recently, however, these methods have been applied to disease models to provide information on the kidney's response to acute and chronic pathologic conditions. The first such studies of disease models were those of Fine, Bricker and their colleagues on the remnant model of chronic renal failure summarized elsewhere in this symposium. We have been interested in examining the effect of acute renal injury on segmental nephron function. In addition, we wish to evaluate methods and maneuvers to ameliorate or prevent the acute renal tubular injury. This, we hope, will allow extention of our observations to clinically relevant situations and therapeutic modalities.