Hydroxyethyleellulose 0.3% Water 93.7% The test agent was applied to the forearm twice daily without occlusion for four days, the other forearm acting as a control. On the fifth day 0.1 ml of 1: 1,000 methacholine (Meeholyl®) was injected intradermally in both forearms as a sweat stimulator; sweat rates were recorded for 15 minutes with a modified Bullard sudorometer (6). A new treated site and a new control site on each forearm was stripped 25 times with cellophane tape; the new baseline water loss was determined to compensate for transepidermal water loss. The injections were repeated at both sites and sweat responses again measured for a 15 minute period. A third site on each forearm was stripped to the glistening layer (up to 45 strips). The new baseline water loss was again established, both sites were challenged with 1: 1,000 methacholine and readings were taken for a 15 minute period.