Clonoamide, a new inhibitor of sterol O-acyltransferase, produced by Clonostachys sp. BF-0131

descriptionPublicationkeyboard_double_arrow_right Article 22 Apr 2015 English Publisher:Springer Science and Business Media LLCJournal:The Journal of Antibiotics, volume 68, pages 615-619 (issn: 0021-8820, eissn: 1881-1469,

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Authors: Keisuke, Kobayashi; Nobuaki, Tsukasaki; Ryuji, Uchida; Yuichi, Yamaguchi; Hiroshi, Tomoda;

doi: 10.1038/ja.2015.37

pmid: 25899123

Clonoamide, a new inhibitor of sterol O-acyltransferase, produced by Clonostachys sp. BF-0131

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Abstract

A new compound designated as clonoamide was isolated from a culture broth of the fungus Clonostachys sp. BF-0131 by solvent extraction, Diaion HP20 column chromatography, octadecylsilyl column chromatography and preparative HPLC as an inhibitor of sterol O-acyltransferase (SOAT). The structure of clonoamide was elucidated as 2-oxo-9E,11E-tridecandienyl acetamide by various spectral analyses including NMR. The compound inhibited SOAT1 and SOAT2 isozymes with IC50 values of 39 and 110 μm, respectively, in a cell-based assay using SOAT1- and SOAT2-expressing Chinese hamster ovary cells.

Related Organizations

Chugai Pharmaceutical Co.
Japan
Kitasato University
Japan

Keywords

Magnetic Resonance Spectroscopy, Fungi, CHO Cells, Microbial Sensitivity Tests, Isoenzymes, Mice, Cricetulus, Anti-Infective Agents, Cricetinae, Microsomes, Acetamides, Chlorocebus aethiops, Fermentation, Macrophages, Peritoneal, Animals, Enzyme Inhibitors, Sterol O-Acyltransferase

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