Muscle gene electrotransfer is increased by the antioxidant tempol in mice
Copyright policy )Muscle gene electrotransfer is increased by the antioxidant tempol in mice
Electropermeabilization (EP) is an effective method of gene transfer into different tissues. During EP, reactive oxygen species (ROS) are formed, which could affect transfection efficiency. The role of generated ROS and the role of antioxidants in electrotransfer in myoblasts in vitro and in Musculus tibialis cranialis in mice were, therefore, investigated. We demonstrate in the study that during EP of C2C12 myoblasts, ROS are generated on the surface of the cells, which do not induce long-term genomic DNA damage. Plasmid DNA for transfection (pEGFP-N1), which is present outside the cells during EP, neutralizes the generated ROS. The ROS generation is proportional to the amplitude of the electric pulses and can be scavenged by antioxidants, such as vitamin C or tempol. When antioxidants were used during gene electrotransfer, the transfection efficiency of C2C12 myoblasts was statistically significantly increased 1.6-fold with tempol. Also in vivo, the transfection efficiency of M. tibialis cranialis in mice was statistically significantly increased 1.4-fold by tempol. The study indicates that ROS are generated on cells during EP and can be scavenged by antioxidants. Specifically, tempol can be used to improve gene electrotransfer into the muscle and possibly also to other tissues.
muscle, Cell Survival, Muscle, skeletal., electropermeabilization, 610, Electroporation., Antioxidants, Cell Line, Cyclic N-Oxides, Myoblasts, Mice, Animals, Muscle, Skeletal, Gene transfer techniques., reactive oxygen species, Reactive oxygen species., info:eu-repo/classification/udc/577.2, electropermeabilization, gene electrotransfer, muscle, tempol, reactive oxygen species, Gene Transfer Techniques, 500, Cell survival., Transfection., Mice, Inbred C57BL, tempol, Electroporation, gene electrotransfer, Original Article, Female, Spin Labels, Antioxidants., Reactive Oxygen Species, Plasmids
muscle, Cell Survival, Muscle, skeletal., electropermeabilization, 610, Electroporation., Antioxidants, Cell Line, Cyclic N-Oxides, Myoblasts, Mice, Animals, Muscle, Skeletal, Gene transfer techniques., reactive oxygen species, Reactive oxygen species., info:eu-repo/classification/udc/577.2, electropermeabilization, gene electrotransfer, muscle, tempol, reactive oxygen species, Gene Transfer Techniques, 500, Cell survival., Transfection., Mice, Inbred C57BL, tempol, Electroporation, gene electrotransfer, Original Article, Female, Spin Labels, Antioxidants., Reactive Oxygen Species, Plasmids
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).26 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!