Although interdisciplinary research has been heralded as the engine of basic discovery for decades, many in the immunology community have been taken back by the recent marriage between microRNA (miRNA) and immunology. MicroRNAs were first discovered by Ambros and colleagues in 1993.1 They are small untranslated RNAs, highly conserved between different eukaryotic species.2, 3, 4, 5 They are encoded by specific genes in the genome, which are controlled at the transcriptional level in a manner similar to protein-encoding genes.2 Following the synthesis of the primary miRNA by RNA polymerase II or III, nuclear processing by the enzyme Drosha produces a primary miRNA transcript which can be shuttled into the cytoplasm2 Final production of the mature miRNA species requires further cytoplasmic processing by an RNase III enzyme called Dicer, producing a 19- to 24-base pair product, capable of being incorporated into the RNA-induced silencing complex. The RNA-induced silencing complex, in turn, is able to use the ‘seed sequence' of the miRNA to recognize complementary mRNA transcripts for degradation or translational silencing. To date, more than 800 human miRNAs have been identified, regulating an estimated 50% of all human genes. Each miRNA appears to regulate the expression of tens to hundreds of genes, thereby functioning as ‘master-switches' that regulate and coordinate multiple cellular pathways in important processes such as embryonic development and oncogenesis, as well as cellular growth and proliferation.2, 3, 4, 5 Inspired by these discoveries, a number of laboratories have recently investigated the potential roles of miRNAs in immune cells. This has led to the unexpected finding that miRNAs are crucial for essentially all major immune processes. For example, Toll-like receptors (TLRs) induce the expression of more than 20 miRNAs (including miR-21, miR-15b, miR-16, miR-23b, miR-27b, miR-30b, miR-30c, miR-125b, miR-146, miR-147 and miR-155), each of which selectively targets a specific group of mRNAs.6, 7, 8, 9, 10 Thus, TLR-induced miR-146a turns off TLR response by degrading IRAK1 and TRAF6 mRNAs that are required for TLR signaling, and mediates lipopolysaccharide tolerance that is crucial for preventing septic shock6, 11 Similarly, TLR4-induced miR-21 blocks TLR4 response by targeting programmed cell death 4, a crucial transcriptional and translational regulator of TLR-induced gene expression12 Knocking down either miR-146a or miR-21, or knocking out dicer, a gene that controls miRNA biogenesis, leads to hyper-activation of TLRs6, 9, 12 Thus, besides the negative regulatory proteins of TLRs,13 TLR-induced microRNAs represent a new class of negative feedback regulators essential for preventing deleterious inflammatory conditions induced by pathogens. In this special issue of Cellular and Molecular Immunology, we provide readers with three expert reviews surveying the most recent advances in this nascent field. Specifically, Mi and colleagues will discuss the roles of miRNAs in the development and function of CD4+CD25+Foxp3+ regulatory T cells and invariant natural killer T cells, two cell subsets that are critical for maintaining immune homeostasis and tolerance. Chan and colleagues will focus on the roles of microRNAs in TLR signaling and endotoxin tolerance; the functions of TLR ligand-induced microRNAs, including miR-146a, miR-155 and miR-132, in regulating immunity and tolerance will be discussed in details. Finally, Chen and colleagues bring us to the mucosal surface, where epithelial cells meet with miRNAs as well as pathogens; miRNAs may modulate epithelial immune responses at multiple levels, including production and release of cytokines/chemokines, expression of adhesion and costimulatory molecules, and feedback regulation of immune homeostasis. However, because miRNA immunobiology is a nascent field, many fundamental questions related to miRNA in immunity have yet to be answered. It is unknown how immune receptors selectively activate some miRNA genes but not others; once activated, it is unclear how miRNAs selectively control immune responses that induce them. TLRs can upregulate >20 miRNAs and downregulate >306, 7, 8, 9, 10 As many of these miRNAs regulated by TLR signaling are also dysregulated in cancer, it is possible that miRNAs form a key link between inflammation and cancer and that the induction of specific miRNAs, including miR-21, by TLRs may be a key step in tumor genesis. Thus, interdisciplinary research will likely continue to be the engine of growth of this nascent field for years to come.